In this article, we’ll explore some of the most commonly used staining techniques in microbiology. Microbiology is the study of microorganisms, including bacteria, viruses, fungi, and parasites. To study these tiny creatures, microbiologists often use staining techniques to visualize and identify different types of microorganisms.
In this comprehensive guide, we will explore the basics of Scanning Electron Microscope , how it works, its applications in various fields, its advantages and limitations, and the potential for future advancements in the technology and its applications.
A Transmission Electron Microscope is a type of electron microscope that uses a beam of electrons to produce high-resolution images of thin specimens. It works by transmitting a beam of electrons through a thin sample and collecting the electrons that emerge on the other side. These electrons are then used to produce an image of the sample.
Electron microscopy is a technique used in scientific research and industry to examine the internal structure of objects and materials on a microscopic scale. Electron microscopes are incredibly powerful tools that allow us to see and study things that are invisible to the naked eye, making them an essential tool for a wide range of applications, from biological research to materials science.
Introduction of Pure Culture-Microbiology :The microbial population in our environment is both large and complex. Many different microbial species are normally present in air, water, soil as well as various parts of human or animal body. To study a specific role played by a specific microorganism in the environment, one must isolate the organism in pure. No reliable physiological determination can be made on mixed cultures of bacteria.
MORPHOLOGICAL STUDY-PURE CULTURES. The identification of an organism is the process of determining its species. A routine systematic study of a pure culture includes morphology, cultural characteristics nutritional requirements, biochemical characteristics, physiological properties, serology, pathogenicity, sensitivity to bacteriophages and genetic characterization.
MAINTENANCE-PRESERVATION OF PURE CULTURES – Associated with the study of pure cultures is the problem of maintaining them for various periods in a viable condition. This is necessary so as to eliminate genetic instability, protect against contaminants and retain its characters. Most bacteriological laboratories usually maintain a large collection of pure cultures, frequently refered to as the stock culture collection.
Methods of isolating Pure Culture.A number of techniques have been employed for the isolation of microorganisms from natural environments. Culture methods employed depend on the purpose for which they are intended. The indications for culture are mainly to